By Harry ed. Sobotka
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Extra resources for Advances in Clinical Chemistry, Vol. 4
Hiatt (H4) has been successful in distinguishing heterozygous from normal individuals by determining the increase in xylulose excretion in the urine following a loading dose of glucuronolactone (L4, T3). 2. FRUCTOSURIA Fructose is found in many plants and is an important portion of dietary carbohydrate. Most commonly it is ingested as free fructose or sucrose. Fructose is not actively transported by the intestinal mucosa, and variable proportions are converted to glucose in the process of absorption; in man, about one-sixth is converted ( M l ) .
This is prepared from “Specpure” calcium carbonate in appropriate amount by dissolution in HCl. Perchloric Acid, HC104, 60% (w/w ). Hydrochloric Acid, HCl (sp. gr. 18). Phosphoric Acid, H3POc (sp. gr. 75). 4 mM KH2P04. Sodium Solutions, 200 mM NaC1; 14 mM NaC1. Magnesium Standards for Plasma, Urine, and Feces. Solutions are prepared by adding 10 ml mixed salt solution A, 10 ml calcium solution, and an appropriate volume of magnesium solution I to 700 ml water in a liter flask. Perchloric acid (50 ml) is now added, and the volume is made up to 1 liter.
Only glucose and galactose are absorbed from the intestine largely by active transport systems (C3). Fructose is partially converted to glucose in the process of being absorbed; approximately 1/6 is converted to glucose in man ( M l ) . The disaccharides lactose, maltose, and sucrose are normally hydrolyzed in the intestine to the constituent monosaccharides, which are then absorbed. It is apparent that a small amount of the ingested disaccharide may be absorbed by normal individuals ( B4, T4).
Advances in Clinical Chemistry, Vol. 4 by Harry ed. Sobotka