Download PDF by Bo Mattiasson, Lei Ye: Advances In Biochemical Engineering Biotechnology

By Bo Mattiasson, Lei Ye

Managed radical polymerization suggestions for molecular imprinting, through Mark E. Byrne
From bulk polymers to nanoparticles, by way of Lei Ye
Post-imprinting and in-cavity functionalization, via Toshifumi Takeuchi
Characterization of MIPs (affinity, selectivity, website heterogeneity…), by way of Richard Ansell
Theoretical features and laptop modelling, via Ian Nicholls
MIPs in aqueous environments, by means of Bin Lu
MIPs for binding macromolecules, via Kenneth J. Shea
Solid section extraction, by means of Ecevit Yilmaz
Sensors, through Sergey A. Piletsky
MIPs for catalysis and synthesis, by means of Marina Resmini
Wastewater therapy, through Bo Mattiasson
MIPs as instruments for bioassays, biotransformation and drug supply, through Meiping Zhao

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Additional info for Advances In Biochemical Engineering Biotechnology Biotransformations

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71 Advances in Biochemical Engineering / Biotechnology, Vol. 63 Managing Editor: Th. J. Carnell 1 Introduction Current regulations governing the introduction of chiral molecules as pharmaceuticals or agrochemicals require that each enantiomer should undergo testing for biological activity prior to development and marketing [1].

In practical terms, a measure of the degree of enzyme inhibition by substrates is of significant value and for this system this has been quantified for a range of aldehydes, ketones and alcohols [70]. It was deduced that ketones and alcohols are competitive inhibitors, whilst aldehydes are noncompetitive inhibitors. Similar progress has also been made for the (S)-Hnl from H. brasiliensis. The purified protein has been overexpressed in a number of systems [39, 44] (see Sect. 3), which adequately fulfills the practical demands required for transformations using this system.

G. biotransformations [1a–c, 2], will be very advantageous. In this respect, the hydroxynitrile lyases (Hnl) and their catalytic production of chiral cyanohydrins may have a significant role to play. The Hnls catalyse the asymmetric addition of hydrogen cyanide (HCN) to the carbonyl moiety of an aldehyde or ketone (Scheme 1) to yield a chiral cyanohydrin (1) (where R1 and R2 R1 = Alkyl or Aryl and R2 = H or Alkyl). Reflecting their role in nature, the cyanohydrin is also cleaved by the HNL to yield HCN and the parent carbonyl compound.

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Advances In Biochemical Engineering Biotechnology Biotransformations by Bo Mattiasson, Lei Ye

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